Composite

Part:BBa_I712070:Design

Designed by: Sasa Jereb   Group: iGEM07_Ljubljana   (2007-10-23)


T7 promoter - RBS -T7 RNA polymerase with NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2708
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 RNA polymerase gene was isolated from BL21 and PCR was performed to attach NLS and RBS.


Source

Genomic DNA of BL21 (E.coli strain)

References